The first one because the scale is straight, let me know if you get it right
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Doctors would let your body's immune system fight off a bacterial infection. If the infection gets worse, then the doctors would proscribe you with some antibiotics so it'll fight off the harmful bacteria.
