Answer:
Heat and pressure
Explanation:
Metamorphic rocks are found deep underground
the highest level which is the 'apex preditor' or in other cases its called a 'tertiary consumer'
Here, given-
homozygous alleles 'a' have a frequency of 0.3.
Also the alleles are in equilibrium in a Hardy-Weinberg population. The frequency of individuals that are homozygous for this allele are= 0.49.
The Hardy-Weinberg equilibrium can be defined as the principle which states that the variation in the genetic makeup of a population remains constant and unchanged till there are no external interferences, influencing the population.
Calculation-

Then to find the frequency of the individuals homozygous for this allele the following formula needs to be used-


Thus, the individuals homozygous for the allele can be calculated by 
Learn more about the Hardy-Weinberg equilibrium here-
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Hi.
After a quick search, I'm pretty positive you're supposed to use the wafting technique.<em>
</em><em />Hope this helps.<em>
</em>
Answer: humoral immune response
The main antibody isotypes in the influenza-specific humoral immune response are IgA, IgM and IgG. Mucosal or secretory IgA antibodies are produced locally and transported along the mucus of the respiratory tract by transepithelial transport and can afford local protection from infection of airway epithelial cells.