Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
The first reason was that his theory of continental drift was just too weak for most geologists to accept. Even though he believed the supercontinent that broke up into different continents moved, he did not have a clear explanation to how the continents moved. The other reason is that some of his explanation clashed with ideas that were widely accepted in the science communities. He used similar fossils from different continents to back up his theory of continental drift. However, at that time, many scientists that had observed similarities in fossils in places like South America and Africa believed there were similar fossils in different continents because of a land bridge that were formed by two continents.
Many science communities believe that land bridges allowed migration of many different species and even people to one place to another by large bodies of water frozen by low temperature known as ice age.
Its d i think im not sure but its most likely
A clade is a group of species that includes an ancestral species and all of its descendents
Answer: 1. Magnetic lines of force start from the North Pole and end at the pole, they're continuos through the body of the magnet
, Magnetic lines of force can meet up with iron more easily than air.
, Two magnetic lines of force can't intersect one another, they have a tendency to contract longitudinally., they have an inclination to expand laterally.
