Answer:
Explanation:
The objective here is to prepare 1 liter of LB + Kan (100 μg/ml final concentration) + Amp (50 μg /ml final concentration) liquid media.
Given that :
the Stock concentration of Amp: 100 mg/ml (since 1 mg/ml = 1000 μg/ml)
it is required that we convert stock concentration in μg/ml since 100 mg/ml = 100000 μg/ml
However, using formula C₁V₁=C₂V₂ (Ampicilin),
where:
C₁ = 100000 μg/ml,
V₁=?,
C₂= 50 μg/ml,
V₂=1000 ml
100000 μg/ml × V₁ = 50 μg/ml × 1000 ml
V₁ = 50 μg/ml × 1000 ml/100000 μg/ml
V₁ = 0.5 ml
Given that:
the Stock concentration of Kan: 25 mg/ml (since 1 mg/ml = 1000 μg/ml)
it is required that we convert stock concentration in μg/ml , 25 mg/ml = 25000 μg/ml
Now by using formula C₁V₁=C₂V₂ (Kanamycin),
C₁ = 25000 μg/ml,
V₁=?,
C₂= 100 μg/ml,
V₂=1000 ml
25000 μg/ml × V₁ = 100 μg/ml × 1000 ml
V₁ = 100 μg/ml × 1000 ml/25000 μg/ml
V₁ = 4 ml
Thus; in 1 lite of Lb+ Kan+Amp preparation;
0.5 ml of Amp & 4 ml of kanamycin is used for their stock preparation.
Finally;
Sterilization step should be carried out in flask (Clean dry glass wares) for media in an autoclave, the container size should be twice the volume of media which is prepared.
I highly believe it would be D
Because yes it does happen above water but it’s not just that, and the other two don’t seem correct. So sorry it’s not correct but I believe it is D
Answer:
1. a chemical reaction in which one substance breaks up into two or more new substances: decomposition reaction
2. a reaction in which two or more substances combine to form a new substance: synthesis reaction
3. the reaction of an acid with a base to form a salt and water: neutralization reaction.
4. chemical compound formed when the negative ions from an acid combine with the positive ions of a base: salt
5. two ionic compounds reacting in solution to form two new compounds, one of which is insoluble: double displacement reaction.
6. a reaction in which an active metal displaces a less active metal or hydrogen from a compound solution (or a nonmetal replaces a nonmetal from a compound in solution): Single replacement reaction
Explanation:
1. Decomposition is a type of chemical reaction in which one reactant gives two or more than two products.
Example: 
2. Synthesis reaction is a chemical reaction in which two reactants are combining to form one product.
Example:
3 and 4. Neutralization is a chemical reaction in which an acid and a base reacts to form salt and water. Salt is formed when cations or positive ions of base combine with anions or negative ions of acid.
Here
is the salt formed by combination of
from base and
from acid.
Example: 
5. A double displacement reaction is one in which exchange of ions take place. The salts which are soluble in water are designated by symbol (aq) and those which are insoluble in water and remain in solid form are represented by (s) after their chemical formulas.
Example: 
6. Single replacement reaction is a chemical reaction in which more reactive element displaces the less reactive element from its salt solution.
Example: 
Answer:
1
Explanation:
since Na has one electron in its outer shell it has to give away that one electron in order to obtain a noble gas structure and chlorine needs to obtain one electron also to became a noble gas so it receives the electron that the sodium gave away.