A plasmid contains a multiple cloning site inside the coding region of the lacZgene and also contains an ampicillin resistance g
ene at a separate locus. When cells are transformed with a successfully recombinant plasmid containing a piece of DNA cloned into the multiple cloning site, what color will the colonies be when grown on an agar plate containing ampicillin and X-gal
As the options are not given, the options are found online and are as follows;
(a.) green (b.) white (c.) colonies will not grow (d.) blue (e.) clear
Out of this, white is the true option because in recombinant plasmid lac Z gene is disturbed and cannot produce beta galactosidase enzyme and there is no hydrolysis of x gal and no blue so recombinant plasmid gives white colonies)
