Straightforward, dependable core facility HLA tissue typing service
Using state of the art genotyping technologies as used in HLA typing for organ transplantation
We work with genomic DNA, Saliva, Whole Blood, or Cryopreserved cells
Detailed results typically sent in 3 weeks
typeHLA Tissue Typing Service Overview
Typing technology options
New Next Generation Sequencing (NGS)
PCR-SSOP using Luminex®
(previously called Tier 1)
HLA Class I loci available
A, B and C
(whole Class I panel reported)
A, B, C
(can be ordered individually)
HLA Class II loci available
DRB1, DPB1 and DQB1
(whole Class II panel reported)
DRB1, DRB3,4,5, DPA1*, DPB1, DQA1*, DQB1
(can be ordered individually)
Resolution of typing data
Fully resolved 4 digit (allelic level) typing with no degeneracy for all samples
4 digit (allelic level) typing but with some degeneracy
Features / Restrictions
Only available for ordering whole Class I panel (3 loci) or whole Cass II panel (3 loci) or whole Class I and Class II panel (6 loci)
Can be ordered for each locus individually
Turnaround time (approximate)
3 weeks
Sample formats accepted
gDNA, Cryopreserved PBMCs/other Cells, Blood, Saliva
Report format
Electronic format (PDF, XLS) via secure webserver
Answer:
Read Below
Explanation:
Nucleotides are A & T and G&C you see in DNA and in RNA T is Replaced by U. The reason they must be balanced between G&C and T&A is because G has to bond with A in DNA and G with C so if there is more G than C that means there is mismatches between the DNA nucleotides same thing for A and T. In RNA you follow the same rule. If we have lets say 27% of our DNA as A we have to have 27% as T leaving 23% as C and 23% as G. If there was lets say 29% T while one 27% A then there was a error in DNA replication and could lead to errors in RNA synthesis if not corrected
Modeling and predicting what is bound to happen in the future using deduction of impossible possibilities
Answer:
Punnett Squares are unable to predict the offspring of an asexual organism because there is only one parent from which the offspring can receive genes from. The whole point of a Punnett Square is the fact that it covers all possible offspring outcomes besides mutations from the usual two parents.
