Answer:
The student forgot to add reverse transcriptase
Explanation:
Reverse transcriptase is an enzyme, also called DNA polymerase or RNA-dependent, responsible for carrying out the transcription process, which corresponds to an “inverted copy”, as opposed to the cellular pattern. In other words, this enzyme polymerizes DNA molecules from RNA molecules, the opposite of what commonly occurs in cells.
The student, exposed in the question, wanted to create cDNA through an isolated and amplified mRNA sample, but this student did not use reverse transcriptase, so it would be impossible for his experiment to have good results.
I think it would be the source of the problem
Answer: death rate, morbidity index, prevalence and frequency of disease.
Explanation: In public health policies, they must respond effectively to the most relevant problem in society, the one they are currently transiting, in this way, with these indices, it is verified whether the problem is really such, the repercussion and the significance in the society. On the other hand, not only respond to the most important problems, but also that everyone has access to the resolution and is maintained over time.
