Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
The correct answer is water.
Water is the most abundant resource in our body and is essential for many bodily functions.
Hope this helps!
Answer:
Cells come from other living cells
All living things are composed of one or more cells
The cell is the basic unit of life.
Answer:
Because hemophilia is an X-linked recessive condition.
Explanation:
As the problem states, hemophilia is an X-linked recessive condition.
- Meaning that in Queen Victoria's female descendants (coded XX) which inherited the Queen's one allele, for hemophilia to appear in them it would be necessary to inherit another hemophillia-carrying allele from the father.
- The Queen's male descendants (coded XY) would always manifest hemophilia if they inherited the Queen's hemophilia allele, as they possesed only one X gene.
