In step 2: Denaturation of the double-strand occurs.
In step 3: Annealing of the primer to the single strands.
In step 4: Extension or elongation takes place in this step.
Explanation:
In the PCR program an enzyme Taq Polymerase is used because it can withstand high temperature without altering its functions.
PCR is required for the amplification of DNA into multiple copies for experimental purpose. The artificial environment is created to form new DNA molecules from the sample in question.
The first step in replication is the opening of the double helix which is done by temperature treatment in PCR. The temperature would be 90 degrees for some 30 sec to two minutes.
The next step of primer annealing would be done at 52 degrees, this is the primer melting temperature.
The elongation of the DNA strand to be synthesized will take place at 72 degrees as Taq Polymerase can withstand that temperature.
Nearly one million copies of DNA will be made after 30 cycles of PCR.
PCR products can be stored at 4 degrees for some two months.
Answer:
The total blood given is 5.5 liters
Thus, total alcohol needed for BAC (blood alcohol content) to exceed 0.1 gram per 100 ml will be 0.1/100 × 5500 = 5.5 grams alcohol.
1 oz = 28.3 grams
28.3 gram water = 28.3 ml
It has 20 percent alcohol by volume,
Therefore, alcohol in one drink will be:
20/100 × 28.3
= 5.66 ml
= 5.66 × 0.79 (density of ethanol)
= 4.47 gram
Thus, no of drinks will be 5.5 g / 4.47 g = 1.2
Hence, 1.2 drinks will make the BAC to exceed 0.1 g / 100 ml in the blood.
Answer:
The cell membrane
Explanation:
One way that a cell maintains homeostasis is by controlling the movement of substances across the cell membrane. The lipid bilayer is selectively permeable to small, nonpolar substances. Proteins in the cell membrane include cell-surface markers, receptor proteins, enzymes, and transport proteins.
Viruses and bacteria infect cells by hijackingreceptors<span> on their surface.</span>
Explanation:
Plasmolysis is the process in which cells lose water in a hypertonic solution. The reverse process, deplasmolysis or cytolysis, can occur if the cell
