Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
It’s where you get a girl and boy together then they have sex
Explanation:
Answer:
shape, pattern, size i took the test
Explanation:
Whattttttt? I can’t see anything
Answer:
la selección natural es el mecanismo principal por el cual la evolución ocurre
Explanation:
Charles Darwin fue un naturalista inglés que propuso la teoría de la evolución o 'descendencia con modificación' mediante el mecanismo de selección natural. Acorde a esta teoría, la selección natural se define como el mecanismo selectivo de reproducción diferencial de los individuos mejor adaptados a su ambiente. La variabilidad es el la materia prima sobre la cual la selección natural actúa, seleccionado aquellos individuos que presentan combinaciones fenotípicas y genotípicas más adaptadas para sobrevivir en un ambiente en particular. Los individuos de una población que son 'superiores adaptativamente' poseen más chances de sobrevivir y reproducirse, y por lo tanto tienen también más probabilidades de transmitir sus genes a la siguiente generación.
