Answer
The answer is A I think...
<span>The nurse would need to consult the beers criteria prior to giving medications to patients who fall in the category of elderly. This list would notify the nurse what medications are considered inappropriate. Incidentally, the list of patients not covered by this list include newborns, toddlers, and teenagers.</span>
Most of the mutations have no effects whatsoever on the organisms but some can be dangerous. There are two types of mutations that cause harm to the organism's ability to survive:teratogen muations are the mutations that form inside the uterus when the fetus is still developing and can even kill it or cause severe malformations that lead to death in the early life. Carcinogen mutations are the ones that lead to the formation of neoplasms(masses of cells that divide uncontrollably, basicly cancer).
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
