Answer:
The answer is E.
Explanation:
In vivo or in vitro evolution (Directed Evolution) is a technique used by genetic scientists to push the change in nucleic acids or proteins in a specific direction to get the end results that they want.
And high-fidelity polymerase is used to get a replica of the target DNA that has less errors.
So the situation given in the question where researchers use a higher-fidelity DNA polymerase in vitro evolution, the mutation rate would most likely be lower because of the high-fidelity DNA polymerase.
I hope this answer helps.
Answer:
they need a high ‘surface to volume’ ratio, which is good for exchanging materials between the inside and outside of cells. But this is probably not really the size-limiting reason, since cells vary enormously in size and surface area to volume ratios.
Explanation:
Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
