A cross between two different lines and/ or genes that differ in two observed traits.
Answer:
The question: Do mice live longer when they are fed high sugar diets?
Hypothesis: There are two hypothesis the null hypothesis which we assume is correct, it states that a high sugar diet will have no effect on the life span of a mouse and an alternate hypothesis that we accept if evidence shows that sugar does increase the life span if mice. It says a high sugar diet does incerease the life span of mice.
Prediction: Assume that the null hypothesis is correct. High sugar diets will not increase the life span of the mice.
Collect data through experimentation. Use some mice as a 'control'. These mice will not undergo any changes but will be kept for comparison. Expose some other mice to high sugar diets and the compare the outcome with the 'control' mice.
Analyzing: From the experiment this you can choose which hypothesis you are going to accept. I.e. null: no change or alternate: there was a change and do some extra research to back up your hypothesis.
Hope this helps :)
Answer:
For the 1st part, the correct answer is option C.
In a polymerase chain reaction, the amplification of DNA template takes place not RNA. So, prior to going into the process, there is a need to initially convert RNA into DNA with the help of an enzyme reverse transcriptase.
For the 2nd part, the correct answer is option D.
The suitable temperature for human DNA polymerase is 37 degrees Celsius, and it gets denatured at greater temperatures. As PCR runs at exceedingly high temperature like 95 degrees Celsius, only Taq DNA polymerase can withstand the temperature. Thus, normal DNA polymerase cannot be utilized in the PCR reactions.
