Convection : Warm air rises and cold air sinks, you have a cycle there :D
You can experience it on the beach shore. Since water has a high heat capacity ( it takes longer to heat up ) and the sand get hot quickly, the air on the shore gets warm and rises. When the air reaches to certain elevation (or meet the temperature of the beach), it gets cooler and sinks, cold air from the beach blown to the shore.
Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer: age, smoking habits, and size/weight
Explanation:
The older you get, the weaker you'll be, so your breathing will be effected.
If you smoke then your lungs will be effected which will ultimately have an affect your breathing.
If you're really heavy (ex. Over weight) then you will get tired more easily, which will effect your breathing rate.
