Question

Outline two disadvantages associated with staining procedures. ​

Answer 1

Preparation of the slides using the paraffin technique can be time-consuming; frozen slides are faster to prepare, but this can affect the resolution, especially when using light microscopy.

Answer 2

Answer:

The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed. It is also used to prepare biological samples for electron microscopy.

Secondly, what are the limitations of simple staining? Disadvantages. It does not give much information rather than the morphological characteristics of bacteria. Through simple staining, we cannot classify a particular type of organism.

Regarding this, what is an example of a negative stain?

In a negative staining technique, an acidic, anionic dye is mixed with a cell sample. The dye changes the color of the background, not the cells, causing the cells to stand out. India ink is the classic example of a negative stain.