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In electrophoresis, the molecules travel between the positive electrode and the negative electrode carried by the current that is generated between them.
As seen in the image below, short fragments, due to their smaller size can travel faster than the longer fragments, so the fragments that have traveled the furthermost are the shortest. (marked E in the image)
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If there is a temperature difference between two objects, the object that has a higher temperature will want to level out its heat. Therefore, it will transfer the heat it needs to the other object if it allows until they are the same temperature.
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