Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
This is an example of Human Genome Project. It was a global logical research extend with the objective of deciding the arrangement of nucleotide base matches that make up human DNA, and of recognizing and mapping the greater part of the qualities of the human genome from both a physical and a practical point of view.
