Question

You have a mixture of gram positive and gram negative cells in culture. You prepare a slide from the culture and gram stain it, but when observing the sample using oil immersion, all cells appear purple. What is the likely technical reason why only purple-colored cells

Answer 1

Answer:

due to imperfections in the staining

Explanation:
if you skip the decolorization phase and therefore not resorb the violet stain from the wall of the gram-negative bacteria, which will not provide a base for the obtaining of safranin stain which should stain the gram-negative bacteria red or pinkish, the gram-negative bacteria will have the same color as the gram-positive, so all the cells will be purple