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I am Lyosha [343]
2 years ago
10

Life is hard *sigh* :/

Biology
1 answer:
wariber [46]2 years ago
3 0

Answer:

sure is

Explanation:

im gonna go to 10th grade after finals thays in 10 days :) someone save me T _T

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Why choose that gene for PCR in the bacterial identification lab?
Reika [66]

Answer:

Background

During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.

Explanation:

Results

Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.

3 0
3 years ago
The distance between Jupiter and the sun is usually measured in astronomical units. Which answer choice below best defines an as
Lostsunrise [7]
An astronomical unit (AU) is the approximate distance from the earth to the sun. The answer is D.
4 0
3 years ago
Read 2 more answers
In an electrochemical cell, q = 0.0010 and k = 0.10. what can you conclude about ecell and e∘cell? in an electrochemical cell, =
Iteru [2.4K]
If there is electrochemical cell where Q = 0.0010 and K = 0.10 then the truth about Ecell and E°cell is,
Ecell is positive and E°cell is negative.
E° = (RT/nF) InQ = (RT/nF) × In(K/Q) = Positive.
Since ( K/Q) = 100 then In(K/Q) = positive
8 0
3 years ago
When is selective breeding okay and when is it not? Provide evidence.
jonny [76]

Answer:

Farmers have been using selective breeding for improving their crop qualities and producing animals with better characteristics.

improved so that mankind can benefit from it.

Selective breeding is not okay on a bigger level where it might eradicate the natural varieties of plants and animals completely.  When done on a larger level, the wild-type traits of plants and animals will change and be removed completely. This will lead to lesser genetic diversity.

5 0
3 years ago
What does the prefix "hetero-" mean?<br>A. Same<br><br>B. Different
disa [49]
I would say different
Like you like different genders
4 0
3 years ago
Read 2 more answers
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