Answer:
A) A transgene integrated into a heterochromatic region of the genome.
Explanation:
This is most likely the reason why the transgene is not expressed in the fifth cell line. It is likely that the transgene was integrated into a heterochromatic region of the genome. Heterochromatin consists of genetically inactive satellite sequences, and many of these are repressed to various extents. Moreover, because this is a tightly packed form of DNA or condensed DNA, it is inaccesible to polymerases and thus not transcribed.
No, cladograms deal with living species.
They are both important because mechanical has to do with physical - cause mechanical means physical, so chewing your mouth is physical (you break your food into smaller pieces physically). And then chemical digestion is when food and saliva mix together. They happen when breaking down food into nutrients - enzymes. Enzymes are important for that process, because they make a nutrient.
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