What is the starting material in transcription?
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Answer:
Explanation:
Proteins, such as RNA (ribonucleic acid) polymerase, were loaded into lane 2. TFIIB and TFIID are two forms of TFII. These proteins attach to the fragment of DNA. Furthermore, these proteins shield DNA from the DNase enzyme, which may trigger DNA to split. As a result of the above findings, we may conclude that the inserting proteins(RNA polymerase, TFIID and TR 8 proteins) cover the area between 500 and 800 bp (base pair) in the DNase I footprinting experiment.
As a consequence, the length duration is 300bp since the promoter area is about 500-800bp and is not digested by DNase after incubation of inserting proteins.
