Answer:
The purpose of ethical guidelines for handling of experimental subjects is to ensure the safety and well-being of all living creatures in an experiment. Ethical guidelines include issues such as human rights, animal welfare, safety compliance with the law, conflicts of interest, health standards.
TRNA stands for transfer-RNA which is used in the process of protein synthesis or translation. tRNA is a unique clover leaf like structure that has 4 arms- amino acid acceptor site (acceptor arm), D loop, T psi C loop and anticodon loop. It also has a small variable loop. The anticodon loop consists of a sequence of 3 bases that is complementary to the 3 bases present of mRNA to be translated. A tRNA for alanine will have an anticodon for alanine while a tRNA for lysine will have an anticodon for lysine. For a tRNA to insert an alanine in place of lysine in a forming peptide, the anticodon and the amino acid acceptor site should be altered so that it accepts lysine in place of alanine and add it to the growing polypeptide.
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