Hi lovely,
The answer you're looking for is C)
The term domesticus is used to determine the type of species a certain animal falls into.
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
Answer:
The correct answer is C the polar head groups of phospholipid,glycolipid and cholesterol face towards the interior of the bilayer.
Explanation:
Plasma membrane contains various lipid molecules such as phospholipid,glycolipid,sphingolipid and cholesterol.The polar head groups of the phospholipid,glycolipid and cholesterol faces the hydrophilic region which is present outside the lipid bilayer and the nonpolar groups faces the hydrophobic region that is present interior of lipid bilayer.
Answer:
Oxygen.
Explanation:
Yeah no thats' all there is to it...
