Where in the human body does the digestive process begin?.
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- 20 cycles of PCR is necessary for the amplification target sequence
Mass is proportional to sequence length.
Let one base pair have mass M.
Thus,
will have mass
And the 3.2kb target will have a mass of
For the total amount of amplified target sequence to constitute 50% (by mass) of the total DNA in the reaction mixture, it has to be amplified to such an extent that the total mass of the amplicon matter is also
PCR amplification happens geometrically, doubling the DNA target with each cycle.
Thus, if the number of cycles in N,
Then,
For more information on cycles of PCR, visit