formation of microspheres might have been the first step toward cellular organization( found this somewhere)
"No, if you can reconstitute an aquaporin in a membrane it will function fine. Aquaporins that actively transport water will not function, but many aquaporins are passive transporters" (https://www.quora.com/Do-aquaporins-require-a-living-cell-in-order-to-function)
Cells differentiate in order to create cells that perform specific functions.
Nucleic acids are long chainlike molecules that carry genetic information which is read in cells to make RNA and proteins by which living things function.