Question

The following diagram shows a DNA template which is 70bp long (spaces indicate 10bp blocks) and the sequence of 4 primers. What size products (if any) would be generated from a PCR with the following primer combinations? Briefly explain your answer.
5’ AAGCCTTGCA TTGACTCGGA GCGCGTATTA GCGTAAGCCT CAGAGTCCGA TTCCAGTCAG CTAGCGCATT 3’

3’ TTCGGAACGT AACTGAGCCT CGCGCATAAT CGCATTCGGA GTCTCAGGCT AAGGTCAGTC GATCGCGTAA 5’

Primer 1 5’CCTTGCATTG3’
Primer 2 5’ GGAACGTAAC 3’
Primer 3 5’ CCGATTCCAG 3’
Primer 4 5’ TGCGCTAGCT 3’

a. primers 1 and 2
b. primers 1 and 3
c. primers 1 and 4
d. primers 2 and 3
e. primers 2 and 4
f. primers 3 and 4

Answer 1

NONE of these DNA fragments can be completely amplified by using these primer combinations. PCR is a molecular biology technique.

What is PCR?

Polymerase chain reaction (PCR) is a technique used in molecular biology to replicate (amplify) a given fragment of DNA.

In this technique (PCR) primers sequences must be used to add nucleotides to the amplified DNA strands during DNA replication.

These nucleotide primers must be sequence complementary to the fragment of DNA desired to be amplified by PCR.

In this case, none of the DNA fragments can be fully generated by using these primers or any of their combinations.

Learn more about PCR here:

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