Answer:
e. None of the above
Explanation:
For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.
i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.
I think the answer may be D. But don’t answer yet, I may be wrong.
velocity= distance / time
your distance is 180 miles
your time is 3 hours
180 divided by 3 = 60
An isolated colony on a selective medium is not considered a pure culture because inhibited organisms might be masked by the colonies. This statement is true.
On the basis of their constitution or usage, culture media can be classified into various groups; these include defined, complex, selective, as well as enrichment medium.
A selective medium consists of dyes or noxious substances/chemicals which inhibit the growth of specific microorganisms but promote the growth of other microbes.
A pure culture is a laboratory culture which contains a single species of microorganism. It is free from other microorganisms.
Since selective media affects the growth of distinct organisms differently, therefore, an isolated colony formed in such type of medium is not considered a pure culture.
To learn more about pure culture here
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