Answer:
42,5 mL
Explanation:
We need to use the serial dilution formula beacuse we start with a stock concentrate solution and we need to prepare a new less concentrated one.
<u>DF in the dilution factor, Vi is the initial volume and Vf is the final volume.</u>
The first step is to have the same measurment unit so we need to convert 345 µg to mg.
we know that 1 µg equals 0,001 g, hence:
now the final volume is 0,345 mg protein/ mL and the inital volume is 15mg protein/mL, both of them are in the same unit so we can use the formula
Now since the question said that we already have 1.0mL of the amylase stock solution we need to subtract that 1.0mL to the 43,5 mg protein/mL
So, we need 42,5 mL of diluting buffer if we want a final concentration of 345 µg protein/mL (0.345 mg protein/mL)
The ANALOG FORECASTING METHOD is known as the oldest method in the forecasting of weather. This kind of method reviews the previous weather events in order to lead to a particular weather event. Hope this helps.
I think it is light so B) light
Answer: etiolation of plant growth in shade, with fast growing cell without chloroplasts. Out of shade, cell differentiate again to produce photosynthesising cells
Explanation: It is a subjective decision as to what is ‘best’. Good examples are plant responses to changes in the nutrient supply.
when soil nitrogen is depleted some plants, such as legumes, grow nodules on their roots, with cells that release chemical signals to attract nitogen fixing bacteria.
Lack of light induces etiolation in many plants. New cells elongate and develop without chloroplasts, with rapid cell division exhibiting gravitropism, extending upward. When they grow beyond the shade area, cell differentiation changes again, to produce photosynthesising cells.
If one strand of a DNA molecule has the sequence of bases 5-ATTGCA-3, the mRNA synthesized following the template will be 3-UAACGU-5.
Nucleotides are added to the growing strand one at a time in the precise sequence dictated by the existing template strand. adenine and thymine are always paired with each other in the Watson-Crick DNA model, and cytosine is always paired with guanine.
The nucleotide sequence of DNA serves as a template for the nucleus, where mRNA is synthesised. The RNA polymerase II enzyme is responsible for this reaction's catalysis and needs nucleotide triphosphates as its substrates. Transcription is what happens in the nucleus when DNA is converted into mRNA. The cytoplasmic synthesis of proteins is controlled by the mRNA. The cytoplasm is where the mRNA that is produced in the nucleus attaches to the ribosomes after being transported from the nucleus. The nucleotide sequence of the mRNA serves as a guide for the assembly of proteins on ribosomes. The cytoplasm receives a "message" from the nucleus via mRNA. The nucleotide sequence of the mRNA, which is complementary to the nucleotide sequence of the messenger RNA, encodes the message.
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