Answer:
D. functional tRNAs have been spliced by splicesomes.
Explanation:
Functional tRNAs are not spliced by splicesomes, they are trimmed with the help of RNAases instead.
In all tRNAs, RNAase P cuts at 5'end so as to produce a monophosphate because a phosphodiester bond consists of only one phosphate and 2 sugar molecules.
After that 3'end processing occurs in which RNAase II and RNAase D remove extra nucleotide sequences at 3' end. CCA is added at 3' end later on with the help of enzyme named as tRNA nucleotidyltransferase.
So, there is no role of splicesomes only RNAases and other enzymes are involved in tRNA processing.
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