Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
<u>Nucleic Acid, and Protein</u> are the class of biomolecule which are the two main ingredients in plant fertilizer.
Biomolecules- A chemical substance that is present in living things is called a biomolecule. These consist mostly of substances with the chemical elements carbon, hydrogen, oxygen, nitrogen, sulfur, and phosphorus. The building blocks of life, biomolecules serve crucial roles in all living things.
Fertilizer- Any product or material given to soil to encourage plant development is referred to as fertilizer. There are many different types of fertilizers, and the majority of them include potassium, phosphorus, and nitrogen (N) (K). In actuality, the package of fertilizers bought in supermarkets lists the N-P-K ratio.
To know more about the Biomolecule, click on the below link,
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3. Replication
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4. The cell's nucleus divides into two new nuclei; One copy of DNA is distributed into each daughter cell.
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5. anaphase
6. Prophase
7. Telophase
8. metaphase
Answer:
The fate of glucose-6-phosphate,glycolytic intermediates and pentose phosphate pathways are described below
Explanation:
Fate of Glucose -6-phosphate
Glucose-6-phosphate undergo dephosphorylation to form glucose when there is an increase demand of glucose in the body.
Glucose-6-phosphate enters into pentose phosphate pathway to synthesize ribose-5-phosphate which is used during denovo pathway of purine nucleotide biosynthesis.
Fate of glycolytic intermediates
Glyceraldehyde-3-phosphate is an important intermediate of glycolysis.The glyceraldehyde-3-phosphate act as a precursor during lipogenesis that deals with the biosynthesis of triacylglycerol.
Fate of pentose phosphate pathway intermediates
Ribose-5-phosphate and NADPH are the important intermediates of pentone phosphate pathway.
Ribose-5-phosphate act as a substrate molecule during the denovo biosynthesis pathway of purine nucleotides.
NADPH act as a reducing agent during fatty acid biosynthesis process.
