Answer: Tetracycline.
Explanation:
A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. <u>Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand</u>. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism <em>Providencia stuartii</em> that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform <em>E. coli</em> cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. <u>So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme</u>. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.
Answer:
1. C. Condenser
2. C. 100
3. d. concentrate light coming from the bulb
Explanation:
The Condenser is the part of a microscope that helps to condense the light rays thereby making the visuals clearer and more efficient. This is possible by the increase in concentration of the rays .
The total magnification of the cells when using the immersion objective of bright field microscope is 100x.
The role of the immersion oil is to concentrate light coming out from the bulb