For a mutation to affect evolution it has to be passed on to all the offspring if that is what you mean.
The nucleus is replicated , therefore a copy of DNA is made
C: lack of water during long periods..
The correct answer is option C, that is, behavioral isolation.
The phenomenon of behavioral isolation takes place when two populations possess the tendency of interbreeding, but exhibit distinctions in courtship rituals or other kinds of behavior. For example, the western and eastern meadowlarks are very identical birds whose habitat overlap in the middle of the United States.
However, the members of the two species will not mate with each other, possibly as they use different songs in order to fascinate males. The eastern meadowlarks will not respond to the songs of western meadowlarks, and vice versa.
