The answer is 3) Other nations saw that Spain could be beaten and they challenged Spanish land claims.
Until this defeat, Spain and particularly its armada were seen as practically invincible, so other nations generally obeyed Spain and didn't stand up against it. However, once it was beaten (and their powerful armada practically destroyed), their place of power was reduced).
he was assassinated by John Wilkes Booth, April 14, 1865, while attending the play Our American Cousin at a theater in Washington, D.C.