Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M

1 answer:

7 0

Based on the dilution formula, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

<h3>How can 50-fold dilution of the enzyme be done?</h3>

The 50-fold dilution of the stock enzyme solution can be done by using the dilution formula to determine the given volume of the stock solution required.

The dilution formula is given below:

C1V1 = C2V2

where:

C1 = Initial concentration of enzyme
C2 = Final concentration of enzyme
V1 = Initial volume
V2 = Final volume

From the given data for the enzyme dilution;

C1 = 1

C2 = 1/50 = 0.02

V1 = x

V2 = 5 ml

Making V1 subject of formula in the dilution formula:

V1 = C2V2/C1

V1 = 0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

Learn more about dilution at: brainly.com/question/24709069

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3 years ago

If 0.896 g of a gas occupies a 250 mL flask at 20°C and 760 mm Hg of pressure, what is the molar mass of the gas?

salantis [7]

Answer:

86.2 g/mol

Explanation:

Before you can find the molar mass, you first need to calculate the number of moles of the gas. To find this value, you need to use the Ideal Gas Law:

PV = nRT

In this equation,

-----> P = pressure (mmHg)

-----> V = volume (L)

-----> n = moles

-----> R = Ideal Gas constant (62.36 L*mmHg/mol*K)

-----> T = temperature (K)

After you convert the volume from mL to L and the temperature from Celsius to Kelvin, you can use the equation to find the moles.

P = 760 mmHg R = 62.36 L*mmHg/mol*K

V = 250 mL / 1,000 = 0.250 L T = 20 °C + 273.15 = 293.15 K

n = ? moles

PV = nRT

(760 mmHg)(0.250 L) = n(62.36 L*mmHg/mol*K)(293.15 K)

190 = n(18280.834)

0.0104 = n

The molar mass represents the mass (g) of the gas per every 1 mole. Since you have been given a mass and mole value, you can set up a proportion to determine the molar mass.

$\frac{?grams}{1 mole} =\frac{0.896grams}{0.0104moles}$ <----- Proportion