Maturation is a threat to internal validity stemming from either long-term or short-term physiological changes occurring within the participants that may influence the dependent variable.
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What are physiological changes?</h3>
Ageing causes physiological changes in all organ systems. Arteriosclerosis develops, blood pressure rises, and cardiac output declines. Lung function is compromised, vital capacity is reduced, and expiratory flow rates are slowed.
Pregnancy causes a number of physiological changes in the mother, such as an increase in fat and total body water, a drop in plasma protein concentrations, particularly albumin, an increase in blood volume, cardiac output, and blood flow to the kidneys, and a decrease in blood pressure.
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