Question

you fuse a sequence that contains a short stretch of hydrophobic amino acids to the c-terminus of gfp.you monitor this protein by fluorescence microscopy to characterize the final localization.coursehero you predict the unmodified gfp protein will be found

Answer 1

The sequence containing short stretch of hydrophobic amino acids fused  to the c-terminus of GFP, will be probably found outside the cell because it has no signal sequence.

GFP, called the Green Fluorescent Protein emits a green fluorescence when exposed to light. The protein was first isolated from a jellyfish Aequorea victoria. In laboratories, the protein is majorly used as a marker to track the movement of functioning of any substance.

Signal sequence are the short stretch oh polypeptides found attached to any protein to determine its target site. These sequences determine the pathway and the function that the protein will follow.

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