Question

you discover a new microbe while working on a citizen scientist project. the microbe is taken to a lab that specializes in placing organisms in their correct phylogenic niche. in order to determine its evolutionary relatedness to other microbes, the lab carries out

Answer 1

SSU rRNA analysis

Arbuscular mycorrhizal (AM) fungus are members of the order Glomales and are difficult to distinguish by hyphal morphology. The fungus present in the host or soil may be effectively identified using PCR. On a few sequences of a variable region in the SSU rRNA gene, several of the primers that have been utilized were developed. We analyzed 51 partial Glo males SSU rRNA gene sequences from 33 glomalean isolates to confirm the specificity of primers of this kind that have been utilized in previous studies and to offer data for future primer design.

Each DNA was confirmed by phylogenetic studies in order to differentiate pollutants from the AM fungus (AMF). 39 species were represented in the 88 sequences from 58 isolates that were analyzed together with sequences from the EMBL database.

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