Question

After you perform gel electrophoresis on your pcr product, you see that one sample has a 200 bp band and the other does not. what can you conclude from these results?

Answer 1

After performing gel electrophoresis on the pcr product, you see that one sample has a 200 bp band and the other does not this shows that 200 bp band is significantly larger than the size of primer dimers unless the primers are 100 bp long, they are not visible.

Gel electrophoresis is the process used to evaluate the success of the PCR reaction.A closer examination of the suitability of agarose gel electrophoresis is done for assessing PCR product yield.

PCR is a series of chemical reactions that take place outside a living cell

Use of 2% agarose gel and 100bp marker and spreading the marker strips properly with 80-90V is done to avoid heating.

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