If we know the sequence of nucleotides within a gene then we can determine the primary structure of the protein with the most accuracy.
The central dogma is the process of transcription and translation combined. Through the process of transcription, the specific arrangement of nucleotides in a gene for the mRNA. In the process of translation, the mRNA makes proteins.
The primary structure of a protein is defined as the specific sequence of amino acids that are linked by polypeptide chains. The arrangement of nucleotides within a gene that codes for specific amino acids will determine the primary structure of the protein. If there is a change in the nucleotide sequence then this can result in the formation of the wrong protein which can be harmful.
Although part of your question is missing, you might be referring to this full question:
If you knew the sequence of nucleotides within a gene, which one of the following could you determine with the most accuracy?
The primary structure of the protein
The secondary structure of the protein
The tertiary structure of the protein
The quaternary structure of the protein
Can't tell
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