Answer: Km = 10μM
Explanation: <u>Michaelis-Menten constant</u> (Km) measures the affinity a enzyme has to its substrate, so it can be known how well an enzyme is suited to the substrate being used. To determine Km another value associated to an eznyme is important: <em>Turnover number (Kcat)</em>, which is the number of time an enzyme site converts substrate into product per unit time.
Enzyme veolcity is calculated as:
![V_{0} = \frac{E_{t}.K_{cat}.[substrate]}{K_{m}+[substrate]}](https://tex.z-dn.net/?f=V_%7B0%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D%7D%7BK_%7Bm%7D%2B%5Bsubstrate%5D%7D)
where Et is concentration of enzyme catalitic sites and has to have the same unit as velocity of enzyme, so Et = 20nM = 0.02μM;
To calculate Km:
![V_{0}*K_{m} + V_{0}*[substrate] = E_{t}.K_{cat}.[substrate]](https://tex.z-dn.net/?f=V_%7B0%7D%2AK_%7Bm%7D%20%2B%20V_%7B0%7D%2A%5Bsubstrate%5D%20%3D%20E_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D)
![K_{m} = \frac{E_{t}.K_{cat}.[substrate]-V_{0}*[substrate]}{V_{0}}](https://tex.z-dn.net/?f=K_%7Bm%7D%20%3D%20%5Cfrac%7BE_%7Bt%7D.K_%7Bcat%7D.%5Bsubstrate%5D-V_%7B0%7D%2A%5Bsubstrate%5D%7D%7BV_%7B0%7D%7D)

Km = 10μM
<u>The Michaelis-Menten for the substrate SAD is </u><u>10μM</u><u>.</u>
I’m am most positive the answer is c. For cat
Answer:
A star begins its life as a cloud of dust and gas (mainly hydrogen) known as a nebula. A protostar is formed when gravity causes the dust and gas of a nebula to clump together in a process called accretion. ... If a critical temperature in the core of a protostar is reached, then nuclear fusion begins and a star is born.
Explanation:
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