Question

Which of the following DNA sequences is one strand of a restriction enzyme recognition sequence? Which of the following DNA sequences is one strand of a restriction enzyme recognition sequence? 5’ GGATCC 3’ 5’ AAACCC 3’ 5’ GGGTTT 3’ 5’ GGGGGG 3’

Answer 1

A: ' 5’ GGATCC 3’ ' is a DNA sequence with one strand of a restriction enzyme recognition sequence.

A restriction enzyme refers to a DNA-cutting enzyme that recognizes particular sites in DNA. Various restriction enzymes create staggered cuts at or near their recognition sites, making ends with a single-stranded overhang. In case when two DNA molecules have matching ends, the enzyme DNA ligase can be used to join them. The restriction endonuclease enzyme (EcoRI) recognizes the 5'-GAATTC'-3 sequence of DNA and develops a single-strand cut between the G & A nucleotides.

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