Question

a dna restriction enzyme cuts dna at a specific sequence. if a restriction enzyme that cuts at the dna sequence gaattc were used on the dna strand below, and the resulting fragments were put through electrophoresis, how many bands would appear on the electrophoresis gel?

Answer 1

Four bands appear in gel electrophoresis. Gel electrophoresis is an experimental method used to separate mixtures of DNA, RNA, or proteins by molecular size.

DNA is negatively charged, so when a current is applied to the gel, the DNA migrates towards the positively charged electrode. Fragments are ordered by size because short DNA strands migrate through the gel faster than long strands. There are some basic steps for performing gel electrophoresis outlined below. 1) pour the gel, 2) prepare the sample, 3) load the gel, 4) run the gel (expose it to an electric field), 5) stain the gel. Gel electrophoresis is a technique for separating biomolecules by size. Separation of these molecules is achieved by placing them in a small pore gel and creating an electric field across the gel

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