The agarose gel's mesh-like structure allows for DNA separation. Larger DNA pieces become stuck and move slowly through the pores, but smaller fragments can pass through the pores quickly.
<h3>What connection exists between the DNA's size and the agar gel's structure?</h3>
Because DNA molecules have a constant mass/charge ratio, they are separated by size in an agarose gel in a way that the distance they travel is inversely correlated to their molecular weight (3).
<h3>What connection exists between the DNA fragments' size and gel electrophoresis appearance?</h3>
The negatively charged nature of DNA causes it to move toward the positively charged electrode when an electric current is supplied to the gel. Because shorter DNA strands pass through the gel more quickly than longer strands, the fragments are organized according to size.
<h3>What connection exists between the size of a DNA molecule and its rate of gel migration?</h3>
DNA fragments are separated based on molecular weight using agarose gel electrophoresis. The distance travelled on the gel correlates inversely with the logarithm of the molecular weight, with smaller fragments migrating quicker than larger ones.
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