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1 year ago

Use the gel below to determine your double stranded segment of DNA

Biology

1 answer:

Nikolay [14]1 year ago

6 0

Sanger sequencing is a method where the DNA is replicated but it adds only one nucleotide to the mix each time. This means that you end with several fragment of the DNA chain that end in that nucleotide, for example, adenine. Theprocess is repeated awith each nucleotide.

After the replication, the DNA is observed in an electrophoresis gel, where the smaller fragments move faster and further, while the bigger fragments remain near the loading point.

We can read the gel from bottom to top to know the sequence of the DNA segment, in this case it would be as fllows: (5' to 3')

5' - CAG GAT ATT GCC GAG GCA TGG ATC CGC CTA AGC TGA CTG GAG AAA ATT CTG ATT AGC GTA TGA ATA - 3'

And the complementary sequence (3' to 5') would be:

3' - GTC CTA TAA CGG CTC CGT ACC TAG GCG GAT TCG ACT GAC CTC TTT TAA GAC TAA TCG CAT ACT TAT - 5'

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What part of the food chain re-uses matter and obtain energy

allochka39001 [22]

Answer:

The organisms at the base of the food chain are photosynthetic.

Explanation:

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2 years ago

The typical size of a bacterium is <br> A. 1 nm<br> B. 10 nm<br> C. 1000 nm<br> D. 1 km

mestny [16]

Answer: 1000 nm

Explanation:

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3 years ago

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3 years ago

Read 2 more answers

Carbon dioxide (CO2) combines with a five-carbon compoundIn one to three sentences, describe what happens during the reduction s

alexandr1967 [171]

We are well aware that there are two stages of photosynthesis:

Light dependent reactions
Light independent reactions/ Calvin cycle/ dark reactions.

Calvin cycle or light-independent reactions:

This comprise the process during which carbon dioxide enters into the leaves of plants and passes through series of steps to form sugar or food. This process depends on the supply of ATP, (the ATP that is formed previously during light dependent reactions).

Now there are three stages of Calvin cycle:

1) carbon fixation
2) reduction
3) regeneration

During the process of carbon fixation CO2 combines with a 5-carbon compound called RuBP or ribulose-1,5-bisphosphate which results in the synthesis of a 6-carbon compound that splits up in to 2 three carbon compounds called phosphoglyceric acid (3-PGA).

Here out focus will be the process of Reduction.

Reduction is the second stage of Calvin cycle during which phosphoglyceric acid (3-PGA) is converted to glyceraldehyde-3-phosphate (G3P) that is a sugar. During the process of reduction, energy in the form of ATP and NADPH are used for the conversion of 3-PGA to G3P. As 3-PGA is reduced to G3P, therefore this process is known as reduction.

After reduction, a series of reactions occur that lead to the synthesis of glucose but since focus of our question was reduction, so you can see more details of the process in attached figure.

Hope it helps!

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3 years ago

What is number 30 please

bogdanovich [222]

Answer:

The answer is D

Explanation:

hope this helps

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3 years ago