Question

Hybridomas, which produce monoclonal antibodies, are made by fusing cells of the immune system with.

Answer 1

Monoclonal antibodies are made by the fusion of cells of the immune system with B lymphocytes and myeloma cells.

What is Hybridoma technology?

• It is one of the best technologies that is used to produce the monoclonal antibody.
• In this hybridoma technology the B lymphocytes that are antibody producing are isolated from a source after the immunization with a specific antigen and then are fused with myeloma cell line to form hybrid cells that are also called as hybridoma cell lines.
• The hybridoma cell lines are then cultured in the laboratory with specific antigen and then the monoclonal antibodies are produced.
• This can be done in in-vivo or in-vitro condition.
• This method is preferred over all because the production of antibodies by this method is good as the antibodies produced are of high purity and highly sensitive and specific.
• These cell lines can also be preserved for a long time.
• Hybridoma technology has resulted in production of different varieties of monoclonal antibodies with specificity with specific antigens as the monoclonal antibodies are produced by single parental B cells.

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