In this case, it is widely known that enzymes are able to take substrates or reactants to their active sites, in order to catalyze a raft of biochemical reactions by which products that are not usually formed under typical conditions are formed, and then allow them to dissociate from the surface of the enzyme. Such combination formed by an enzyme and its substrates is called the enzyme–substrate complex which is further taken to the isolated product.
Biogeographic isolation affects the changes in gene pools that result from which organisms are breeding -is how biogeographic isolation allows for the change of gene pools over ( time )