Option <u>(c.) the purity of the protein</u>, will be the right one.
Sodium Dodecyl Sulphate provides a negative charge to each protein as a function of their molecular size. It is an anionic detergent, as well as it is there to determine the relative abundance of major proteins.
After denaturing, proteins get unfolded and gets coated with SDS detergent molecules. The SDS-PAGE technique is used for the separation of protein based on their molecular size/weight/mass.
It is so because as it identifies the proteins by its molecular weight. Therefore, SDS is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight, the SDS-PAGE is a technique.
To learn more about SDS-PAGE here
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Orthopneic
The client's paroxysmal dyspnea was probably caused by sleeping in bed with the legs at the level of the heart; this position increases venous return from dependent body areas, increasing the intravascular volume. Sitting up and leaning forward while keeping the legs dependent slows venous return as well as increases thoracic capacity. Although the contour position elevates the client's head, it does not place the legs in a dependent enough position to substantially decrease venous return. The recumbent position is contraindicated. Venous return increases when the lower extremities are at the level of the heart. Also, the pressure of the abdominal organs against the diaphragm decreases thoracic capacity. The Trendelenburg position is contraindicated. Venous return increases when the lower extremities are higher than the level of the heart. Also, the pressure of the abdominal organs against the diaphragm decreases thoracic capacity.
