Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
1000 gram
Explanation:
because mass is constant everywhere
Answer:
There are four laws of thermodynamics that define fundamental physical quantities (temperature, energy, and entropy) and that characterize thermodynamic systems at thermal equilibrium.
Explanation:
Answer:
Number of moles = 0.92 mol
Explanation:
Given data:
Mass of CaSO₄ = 125 g
Number of moles of CaSO₄ = ?
Solution:
Formula:
Number of moles = mass/ molar mass
Molar mass of CaSO₄:
Molar mass of CaSO₄ = 40 + 32+ 16×4
Molar mass of CaSO₄ = 40 + 32+ 64
Molar mass of CaSO₄ = 136 g/mol
Number of moles:
Number of moles = mass/ molar mass
Number of moles = 125 g/ 136 g/mol
Number of moles = 0.92 mol
CO2 ; H20- They are the only ones that, on both sides, combined with another element and bonding of atoms
