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Jet001 [13]
3 years ago
14

The mechanism of chymotrypsin can be viewed as a two-step process, acylation of the enzyme active site followed by a deacylation

reaction. The observation of "burst" kinetics in rapid kinetic studies of the hydrolysis of p-nitrophenylphosphate by chymotrypsin is due to:_______
A. the rate of the acylation reaction being faster than the deacylation reaction.
B. the rates of acylation and deacylation being equal.
C. the rate of the acylation reaction being slower than the deacylation reaction.
Chemistry
1 answer:
gtnhenbr [62]3 years ago
7 0

Answer:

A. the rate of the acylation reaction being faster than the deacylation reaction.

Explanation:

Chymotrypsin belongs to a class of enzymes known as proteases; enzymes that catalyse the cleavage of peptide bonds by hydrolysis.

The mechanism of chymotrypsin catalysis occurs in two distinct phases; (1) an acylation phase where the peptide bond is cleaved and an ester linkage is formed between the peptide carbonyl carbon and the enzyme, (2) a deacylation phase where the ester linkage is hydrolyzed and the non-avylated enzyme is regenerated.

In studies by B.S. Hartley and B.A. Kilby in 1954 of chymotrypsin hydrolysis of the ester p-nitropheylacetate, as measured by the release of nitrophenol, it was discovered that it proceeded with a burst before leveling of to a slower rate. This burst was due to a rapid acylation of all the enzyme molecules with a slow deacylation limiting the turnover of the enzyme.

Similarly, the observation of burst kinetics in rapid kinetic studies of the hydrolysis of p-nitrophenylphosphate by chymotrypsin is due to the initial phase of acylation proceeding much faster than the later phase of deacylation of the enzyme.

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The same heat transfer into identical masses of different substances produces different temperature changes. Calculate the final
myrzilka [38]

Answer: final temperatures will be

a) water 21 C

b) concrete  20.005 C

c) steel   20.008 C

d) mercury  53 C

Explanation:

Change in temp dT = dH / (mass x specific heat)

Specific heat of these materials can be found from many sources:

water = 1 kcal / kg C

concrete = 210 kcal / kg C

steel = 114 kcal / kg C

mercury = 0.03 kcal /kg C

So dT (water) from 1 kcal heat into 1 kg water = 1 kcal / (1 kg x 1 kcal/kg C) = 1 C therefore the final temperature is 20 + 1 = 21 C

But dT (steel) = 1 kcal / (1kg x 114 kcal/kg C) = 0.008 C so the final temperature is 20 + 0.008 = 20.008 C

The results for concrete and mercury are calculated in the same way

7 0
3 years ago
Can somebody Answer number 23 please it’s for a exam
qaws [65]

Answer:

I think everything that's there is correct.

Explanation:

Bleach with pH of about 12.5 would be a strong base.

Milk with pH of 6.5 and Blood with pH around 7 would be neutral.

Battery acid with pH below 1 is strong acid and orange juice is weak acid.

8 0
3 years ago
Which of the following statements is NOT true about practice?
Reil [10]
Where is the following statements??
7 0
2 years ago
Read 2 more answers
After reading the list of physical properties above, you realize that they are describing a substance that is a
Alecsey [184]

The answer is B

Hope this helps!

5 0
3 years ago
A 3.06 gram sample of an unknown hydrocarbon with empirical formula CH2O was found to contain 0.0170 moles of the substance. Wha
Yanka [14]

Answer:

180 amu

C₆H₁₂O₆

Explanation:

Step 1: Determine the molecular mass of the compound

The sample has a mass (m) of 3.06 g and it contains (n) 0.0170 moles. The molar mass M is:

M = m/n = 3.06/0.0170 mol = 180 g/mol

Then, the molecular mass is 180 amu.

Step 2: Determine the molar mass of the empirical formula.

M(CH₂O) = 1 × M(C) + 2 × M(H) + 1 × M(O)

M(CH₂O) = 1 × 12 g/mol + 2 × 1 g/mol + 1 × 16 g/mol = 30 g/mol

Step 3: Determine the molecular formula

First, we will determine "n" according to the following expression.

n = molar mass molecular formula / molar mass empirical formula

n = 180 g/mol / 30 g/mol = 6

The molecular formula is:

n × CH₂O = 6 × CH₂O = C₆H₁₂O₆

5 0
2 years ago
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