Messenger RNA (mRNA) molecules carry the coding sequences for protein synthesis and are called transcripts; ribosomal RNA (rRNA) molecules form the core of a cell's ribosomes
The answer is false.
Reducing carbon footprint has no effect on carbon emissions. Carbon emission is reduced by lower carbon footprints. As you read national geographic,China and USA are doing nothing to help. Instead, china tripled their coal used recently.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
After another enzyme (a DNA helicase) has seperated two orginal strands of DNA, the polymerase moves along the template strand and polymerizes free nucleotides into a new antiparallel strand. ... DNA ligase joins pieces of DNA together, mainly joins Okazaki fragments with the main DNA piece.
